Ate reading frame were verified by DNA sequencing. All cells in the study were transfected using Lipofectamin2000 (Invitrogen, Carlsbad, CA) according to the manufacturer's protocol. Gene expression profiling and Xenobase analysis Total RNA from TUSC2-transfected and vector-transfected MPM H2595 cell line were isolated with Qiagen RNeasy kit, analyzed for quality using an Agilent Bioanalyzer and h